Journal: Journal of Lipid Research

Article Title: Function and distribution of circulating human PCSK9 expressed extrahepatically in transgenic mice

doi: 10.1194/jlr.m800542-jlr200

Figure Lengend Snippet: Fig. 2. Effects of overexpressed human PCSK9 on LDLR protein levels in transgenic mice fed with chow diet. A: hPCSK9 expression markedly diminished liver LDLR proteins. Liver extracts from chow fed mice were pooled for immunoblot analysis. n = 3 for each group. B: hPCSK9 expression reduced kidney LDLR protein levels and did not alter adrenal LDLR proteins. Tissue extracts were pooled from chow diet fed wild-type and hPCSK9 trangenic mice as described in Materials and Methods and were subjected to immu- noblot analysis. n = 3 for each group. LDLR, LDL receptor; PCSK9, proprotein convertase subtilisin/kexin type 9.

Article Snippet: Mouse LDLR protein was detected with a goat anti-mouse LDLR antibody (R and D Systems Cat# AF2255), and human PCSK9 protein in transgenic mice was detected using antibody against V5-tag (Sigma). joined with transgenic mice ( 18 ).

Techniques: Transgenic Assay, Expressing, Western Blot